in the beginning “开始时”,直接用作时间状语,不接of
at the beginning (of) “在……开始时”,后面必须接of,如at the begining of the class
from the beginning “从一开始就……”强调持续
to begin with只能用在一句话的开头,表示首先要讲什么,例如：To begin with,i want to extend my thanks to my parents. 首先,我想感谢我的父母.