英语翻译
The sample digest was then purified until a minimal protein
interface was obtained by a series of equal volume
extractions of 0.1 M Tris pH8 equilibrated phenol,phenol/chloroform/isoamylalcohol (24:24:1) and chloroform/isoamylalcohol (24:1).0.2 volumes of 10M ammonium acetate and 2 volumes of absolute ethanol
were added to precipitate the DNA from the final aqueous phase.
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