动物组织DNA的提取及核酸浓度的测定
为什么要测310nm处的吸光值?细胞裂解液和沉淀液中的EDTA 有什么作用?氯化钠和醋酸钠有什么作用?
人气:252 ℃ 时间:2020-03-28 01:14:50
解答
DNA concentration is measured by OD260,protein usually is OD280.Some proteins have peak absorbency around 310 nm.Therefore it can be used for judging protein contamination.EDTA is able to chelate Mg2+...
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