利用真核基因mRNA的3`端poly(A)尾设计 oligodT12MN的锚定引物,
mRNA 5’________ AAAAAAAAAAAA…. 3’
cDNA 3’________ C TTTTTTTTTT….5’
cDNA 3’________ A C TTTTTTTT….. 5’
cDNA 3’________ G G TTTTTTTT….5’
cDNA 3’________ T A TTTTTTTT….. 5’
这是以前上课时老师告诉我们的实验原理.其实我还没理解呢.