Abstract:With the development of the bioinformatics,EST plays an importent role in nuclear marker-developing,new gene isolation and identification, analysis of gene expression profile,anotation ot genome fuctions, gene silico cloning and so on. This paper introduces the principle of EST technology, technical route and the research in genetic map, physical map, isolating and identifying new gene,gene differential expression, the research in comparative genomics and the general situation of the DNA wafer preparation. This paper sumarizes the research status of EST,and makes an outlook to EST.
Keywords:genome; gene; research status; EST.