and和with的主要区别是词性不同,and是连词,with是介词.
and的用法：用于连接并列的名词,并列的形容词,并列的数词,并列的动词,并列的句子.
例如：
Boys and girls are playing on the beach.男孩和女孩在沙滩上玩耍.
She is beauitful and smart.她既漂亮又聪明.
Two and three makes five 2加3等于5
She ran and said"look out! 她边跑边说 “小心”
I want to be a teacher and he want to be a scientist.我想成为老师,他想成为科学家.
with的用法有很多,当表示”和“的意思的时候,只能放在句中接名词或代词.
例如：
He lived with his brother.他和哥哥住在一起.
I want to make friend with him.我想和他交朋友.