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The introduction of Guangzhou Huali horticultural varieties. A Buddism godness Guanyin Lotus (Herba MonachosoriHenryi) as experimental materials, the explants and the disinfection time outside the plant body culture survival rate and the rate of pollution. At the same time also studied the effect of culture medium on the induction of bud proliferation, to further understand the effects of hormone concentrations and mineral elements on the growth of test materials. The results show that, the explant sterilization time is 48min when the survival rate was higher and the contamination rate, lower mortality rate, the most suitable for seed production; reduce the concentration of NAA, 6-BA concentration, reduce the mineral element concentrations, the inhibitory effect on the proliferation coefficient were increased, the 6-BA and IAA combination on a Buddism godness Guanyin lotus don't shoot proliferation, the optimal medium was MS+6-BA 3 mg/L+NAA 1 mg/L.