关于像素和分辨率的问题（英文）
拜托认真作答 我会给很高的分的
You are working with this microscope configuration:
@ Objective:50X NA 0.85
@ Ocular:10X
@ Relay Lens:Adjustable from 0.1X to 1.0X in steps of 0.1X,set initially at 0.5X
@ Image Detector (CCD Array) of 1024 X 768 pixels spaced 13.2 μm apart
@ You are using a green filter in your system which provides illumination of about 530 nm wavelength
You also have available in your laboratory the following additional components which are compatible with your microscope:
@ Objectives:10X,NA 0.25,20X NA 0.5,100X Oil immersion,NA 1.3.
@ Oculars:1X,2.5X,5X,15X,20X
Questions:
a) What is the resolution of the objective lens,expressed in μm in the object plane (the stage of the microscope)?
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b) What is the separation of two features just at the resolution limit of the microscope when projected onto the ccd array?
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c) Using a factor of 2.3 for the Nyquist criteria,will the image be undersampled,ideally sampled,or oversampled at the CCD array?
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d) Assume that you need at least the resolution available from your 50X objective.Selecting from the additional components above,what combination of components would allow you to achieve nearly ideal resolution at the image plane (CCD) which satisfies the Nyquist criteria?(There are a number of correct solutions to this question.You need only provide one.
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e) What would be one advantage of setting up your microscope as you describe in ‘d’ above,compared to the original configuration?
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