Cell apoptosis and tumor occurrence and development are closely related, it for the maintenance of tissues and organs of normal morphology and function plays an important role in the tumor, treatment and prevention in the process of great significance.
Because the essence herbal has small toxic and side effects, and therapeutic effect is stable and a series of advantages, therefore, is committed to the Chinese herbal medicine screening of anticancer drugs has become the research direction of new century. As a result of resveratrol has antibacterial, anti-inflammatory, the resistance of cancer cells to grow wait for a characteristic, therefore in the treatment of cancer is of great scientific research prospect.
Objective : this experiment in primary human lung adenocarcinoma A549cells as the research object, in order to understand resveratrol induces lung cancer A549 cell apoptosis. Methods: by inverted microscopy, fluorescence microscopy to observe the effects of different concentrations of resveratrol effects on A549cells in 24h,48h and 72h cell growth pattern, at the same time, using the trypan blue dye exclusion assay for the detection of resveratrol on A549 cell growth inhibition rate, and then determine the value of IC50, and cell apoptosis, and then through the flow cytometry in cell cycle and cell mitochondrial membrane potential change. Results show that resveratrol effects in human lung cancer cell A54948h show different degrees of apoptosis traits, while displaying the dose-effect relationship. Resveratrol induced apoptosis of A549in 48h optimal concentrations were 110µ g/mL,80 µ g/mL, and the IC50values were 108.002µ g/mL,77.462 µ g/mL. After the final flow cytometry results, resveratrol may be A549 cell lag stops at S phase and G2 phase, and the mitochondrial membrane potential decreases with concentration. Conclusion: Resveratrol induces apoptosis in human lung adenocarcinoma A549cell apoptosis, the values of IC50were 108.002μ g/mL,77.462 μ g/mL. Mitochondrial membrane potential decreases with increasing concentration. And cell cycle arrest in G2 phase and S phase, and inducing cell apoptosis.我还问了一个类似的问题，你把这个答案粘过去，我把那个分也给你~