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The determination of starch contents was carried out followingthe method of Zhao et al.(2008).After addition of 5 ml of HCl (0.33 M),1 g of grain powder was kept in a boiling water bath for 10 min and 0.5 ml of 30% (m/v) ZnSO4 was added and mixed thoroughly to deposit the grain protein from cooled HCl reaction mixtures; 0.5 ml of 30% (m/v) k3 [Fe (CN)6] was then added and mixed by shaking.The reaction mixtures were adjusted to a volume of 20 ml,then shaken up and filtrated.Starch was then hydrolysed completely into glucose,which was determined by an automatic recording polarimeter (WZZ-2A; SPOIF,Shanghai,
China) using polarimetric analysis at 20–25\2C.Starch content (%) = [(20 9 a)/aD 9 L 9 M (1-H)] 9 100,
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人气:470 ℃ 时间:2020-06-02 13:35:25
解答
淀粉含量的测定方法是按照赵等人写的(2008)论文中的方法实施的.首先添加5ml的HCI(0.33M),然后把1g的谷物粉末放在沸水盆中浸泡10分钟,同时添加0.5ml的浓度为30%(m/v)的ZnSO4进行完全混合,把谷物蛋白质从冷却的HCI...
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