典型程序： 94℃,5min; (94℃,30s；55℃,30s；72℃,1min) x30；72℃,5min.典型体系：10X buffer（含Mg2+）,5ul；2.5mM dNTP,2ul；10uM primer1, 1ul；10uM primer2, 1ul；模板,2ul；Taq,0.5ul；ddH2O,38.5ul...